描述
| 品牌 | 品名 | 产品型号 | 订货号 |
| PHILIPS | 模块 | SCM040 | 940860010011 |
| 产地 | 可售卖地 | 是否进口 | 保修期 |
| 美国 | 全国、国外 | 是 | 一年 |
| 发货地点 | 发货方式 | 使用方式 | 适用行业 |
| 厦门 | 顺丰快递 | 调试安装 | 电厂钢厂水泥厂船舶造纸 |
| 服务优势 | 国外进口 、 备货供应、 | 价格合适、品质可靠 | 图片仅供参考实物为准 |
| 产品特点 | 一手货源、 按型号供货 | 售后有保障 | 何经理1805988-4797 |
QUANTITY: The following components are included:
500 mL Neural Stem Cell Basal Medium (Part No. SCM003)
5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361)
10 µg Basic FGF, lyophilized (Part No. GF003-10UG)
DESCRIPTION: MilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component
system that is convenient and easy to use. The Neural Stem Cell Basal Medium is a
defined serum-free medium that has been optimized for the growth and in vitro
differentiation of neural stem cells derived from rodents. Basic Fibroblast Growth Factor
(FGF-2, bFGF) is provided separately, which when added to the Neural Stem Cell Basal
Medium (SCM003) allows for the growth and proliferation of rat neural stem cells.
Withdrawal of FGF-2 from the Neural Stem Cell Basal Medium will result in the
spontaneous differentiation of rat neural stem cells. Puromycin Solution is provided
separately to help maintain the expression of the GFP labeled transgene.
QUALITY CONTROL: Each lot of Neural Stem Cell Basal Medium is tested for:
Sterility Testing: Negative
Osmolarity: 299
PH: 7.4
MATERIALS REQUIRED
BUT NOT SUPPLIED:
• MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
(Millipore Catalog No. SCR080)
• Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
• Poly-L-ornithine (Sigma Catalog No. P3655)
• Laminin (Millipore Catalog No. CC095)
• Accutase™ (Millipore Catalog No. SCR005)
PREPARATION OF
COATED PLATES:
We recommend coating the tissue culture plastic- or glasswares that are used to culture
rodent neural stem cells with poly-L-ornithine and laminin. The following procedure is
recommended:
1. Prepare stock solutions of poly-L-ornithine (10 mg/mL) by dissolving poly-L-ornithine
in sterile water. The stock solution should be stored at -20°C or -80°C.
2. Dilute poly-L-ornithine with water from the stock concentration (10 mg/mL) to yield:
a. 10 µg/mL for polystyrene plates
b. 50 µg/mL for glass plates
